The hospital ia a health service that operates for 1x24 hours and provides inpatient care for 3 shifts. The workload of nurses is quite high so that the level of nurse fatigue is different for each shift. Objective:To determine the relationship between work shifts and work fatigue of nurses in the inpatient ward of the Bandar Lampung Adventist Hospital. Method: The research method is a descriptive correlation with a cross sectional study approach and the sample is taken using a purposive sampling technique. Collecting data using a work fatigue questionnaire given to inpatient nurses and analyzed with the Chi-Square test. Results: From each shift, the highest number of nurses was found, namely 43 nurses who were not tired. The statistical results show that the value of p=0,683 or p>0,05 means that there is no significant relationship between work shifts and fatigue. Recommendation: The next researcher should compare the level of fatigue across various departments and consider several variales such as age, sex, and marital status. Implication: The existence of this research is expected that the level of fatigue of nurses can be known, as well as a reference for management to pay attention to the fatigue level of nurses.